D-Titin

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D-Titin

Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin, and show that it encodes a two-megadalton protein with significant colinear homology to the NH(2)-terminal half of vertebrate titin. Mutations in D-Titin cause chro...

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Mechanoenzymatics of titin kinase.

Biological responses to mechanical stress require strain-sensing molecules, whose mechanically induced conformational changes are relayed to signaling cascades mediating changes in cell and tissue properties. In vertebrate muscle, the giant elastic protein titin is involved in strain sensing via its C-terminal kinase domain (TK) at the sarcomeric M-band and contributes to the adaptation of musc...

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Clash of the titin

In This Issue In This Issue Clash of the titin he sarcomere is more than a complicated piece of structural machinery. According to new results by McElhinny et al. (page 125), a building block of striated muscle cells called MURF-1 may also indirectly regulate gene expression. MURF-1 localizes to sarcomeres thanks to its interaction with titin, a major structural component of the muscle sarcomer...

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D-TITIN: a Giant Protein with Dual Roles in Chromosomes and Muscles

Previously, we reported that chromosomes contain a giant filamentous protein, which we identified as titin, a component of muscle sarcomeres. Here, we report the sequence of the entire titin gene in Drosophila melanogaster, D-Titin , and show that it encodes a two-megadalton protein with significant colinear homology to the NH 2 -terminal half of vertebrate titin. Mutations in D-Titin cause chr...

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Drosophila D-titin is required for myoblast fusion and skeletal muscle striation.

An ethylmethane sulfonate (EMS) mutagenesis of Drosophila melanogaster aimed at discovering novel genes essential for neuromuscular development identified six embryonic lethal alleles of one genetic locus on the third chromosome at 62C. Two additional lethal P element insertion lines, l(3)S02001 and l(3)j1D7, failed to complement each other and each of the six EMS alleles. Analysis of genomic s...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 2000

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.151.3.639